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产品名称 TopoGEN | TG2020-1 | Decatenated kDNA
目录号 TG2020-1
别名 Decatenated kDNA
外观 见COA
分子量 N/A
CAS N/A
溶解度
存储条件 见COA
保存时间 见COA
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TopoGEN | TG2020-1 | Decatenated kDNA


品名:Decatenated kDNA

货号:TG2020-1

品牌:TopoGEN


产品描述

Assays employing crude extracts for topo II activity based upon relaxation of supercoiled DNA can be complicated due to the presence of topo I in partially purified fractions. Additional complications arise with contaminating nuclease activity (due to Mg++) which degrade or nick the supercoiled substrate. These problems can be avoided by using a catenated DNA substrate prepared from the kinetoplast of the insect trypanosome Crithidia fasciculata. KDNA is an aggregate of interlocked DNA minicircles (mostly 2.5 kb) that form extremely large networks of high molecular weight.

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