| 产品名称 | TopoGEN | TG1017 | Topoisomerase I Hi-Loading Inhibition Kit |
|---|---|
| 目录号 | TG1017 |
| 别名 | Topoisomerase I Hi-Loading Inhibition Kit |
| 外观 | 见COA |
| 分子量 | N/A |
| CAS | N/A |
| 溶解度 | |
| 存储条件 | 见COA |
| 保存时间 | 见COA |
| 备注1 | |
| 备注2 |
| 目录号 | 规格 | 价格 | 库存状态 | |
| TG1017 | 500 Units; 1000 Units | 咨询 | 咨询 |
TopoGEN | TG1017 | Topoisomerase I Hi-Loading Inhibition Kit
品名：Topoisomerase I Hi-Loading Inhibition Kit
货号：TG1017
规格：500 Units; 1000 Units
品牌：TopoGEN
产品描述
This kit is designed to allow the customer to screen for agents (drugs, natural products, small molecules, synthetics) that block the catalytic activity of human topoisomerase I (topo I). The use of high stoichiometric ratios of topo I:DNA induces cleavage complexes without the need to use topo I poisons (camptothecin) which facilitates detection of catalytic inhibitors of topoI. The assay (see ref 1) is based on the finding that topo I acts in a cooperative manner on DNA template, whereby it tends to display clustered activity (at neighboring sites, see ref 3); thus, at high ratios of enzyme:DNA, it is relatively easy to detect nicking by topo I in the absence of topo I poisons like camptothecin and its various congeners.
