| 产品名称 | Imanis Life Sciences | CL133 | Hepa1-6-Fluc-Neo/eGFP-Puro |
|---|---|
| 目录号 | CL133 |
| 别名 | Hepa1-6-Fluc-Neo/eGFP-Puro |
| 外观 | 见COA |
| 分子量 | N/A |
| CAS | N/A |
| 溶解度 | |
| 存储条件 | 见COA |
| 保存时间 | 见COA |
| 备注1 | |
| 备注2 |
| 目录号 | 规格 | 价格 | 库存状态 | |
| CL133 | 咨询 | 咨询 |
Imanis Life Sciences | CL133 | Hepa1-6-Fluc-Neo/eGFP-Puro
品名：Hepa1-6-Fluc-Neo/eGFP-Puro
货号：CL133
品牌：Imanis Life Sciences
产品描述
Species：Mouse
Cell Type：C57/L hepatoma
Transgene：Firefly luciferase (Fluc);Enhanced green fluorescent protein (eGFP)
Selection Gene：Neomycin (Neo);Puromycin resistance (Puro)
Hepa1-6-Fluc-Neo/eGFP-Puro is a polyclonal population of the hepatoma Hepa1-6 cell line (ATCC® CRL-1830™). To achieve stable reporter expression in the polyclonal population, parental Hepa1-6 cells were transduced with LV-Fluc-P2A-Neo (LV011) and LV-eGFP-PGK-Puro (LV031) and selected using G418 and puromycin. LV-Fluc-P2A-Neo encodes the firefly luciferase (Fluc) cDNA linked to the neomycin resistance gene (Neo) via a P2A cleavage peptide under the spleen focus-forming virus (SFFV) promoter. LV-eGFP-PGK-Puro encodes the enhanced green fluorescent protein (eGFP) cDNA under the SFFV promoter and the puromycin resistance gene (Puro) under the phosphoglycerate kinase promoter (PGK).
