| 产品名称 | Imanis Life Sciences | CL153 | EMT6-eGFP-Puro |
|---|---|
| 目录号 | CL153 |
| 别名 | EMT6-eGFP-Puro |
| 外观 | 见COA |
| 分子量 | N/A |
| CAS | N/A |
| 溶解度 | |
| 存储条件 | 见COA |
| 保存时间 | 见COA |
| 备注1 | |
| 备注2 |
| 目录号 | 规格 | 价格 | 库存状态 | |
| CL153 | 咨询 | 咨询 |
Imanis Life Sciences | CL153 | EMT6-eGFP-Puro
品名：EMT6-eGFP-Puro
货号：CL153
品牌：Imanis Life Sciences
产品描述
Species：Mouse
Cell Type：Mammary carcinoma
Transgene：Enhanced green fluorescent protein (eGFP)
Selection Gene：Puromycin (Puro)
This is a polyclonal population derived from the EMT6 mammary carcinoma cell line (ATCC® CRL-2755™). Parental EMT6 cells were transduced with LV-eGFP-PGK-Puro (Imanis #LV031), encoding the enhanced green fluorescent protein (eGFP) cDNA under the spleen focus-forming virus (SFFV) promoter and the puromycin resistance gene (Puro) under the phosphoglycerate kinase (PGK) promoter. High eGFP expressing cells were selected using puromycin. The lentiviral vectors are self-inactivating (SIN) vectors in which the viral enhancers and promoters have been deleted. Transcription inactivation of the LTR in the SIN provirus increases biosafety by preventing mobilization by replication competent viruses and enables regulated expression of the genes from the internal promoters without cis-acting effects of the LTR1. *The ATCC trademark and trade name and any and all ATCC catalog numbers are trademarks of the American Type Culture Collection. This cell line has been tested for mycoplasma and found negative for mycoplasma contamination. The parental EMT6 cell line has been authenticated and certified free of interspecies cross contamination by short tandem repeat (STR) profiling.
