| 产品名称 | Imanis Life Sciences | CL109 | B16F10-mNIS-Neo/iRFP-Puro |
|---|---|
| 目录号 | CL109 |
| 别名 | B16F10-mNIS-Neo/iRFP-Puro |
| 外观 | 见COA |
| 分子量 | N/A |
| CAS | N/A |
| 溶解度 | |
| 存储条件 | 见COA |
| 保存时间 | 见COA |
| 备注1 | |
| 备注2 |
| 目录号 | 规格 | 价格 | 库存状态 | |
| CL109 | 咨询 | 咨询 |
Imanis Life Sciences | CL109 | B16F10-mNIS-Neo/iRFP-Puro
品名：B16F10-mNIS-Neo/iRFP-Puro
货号：CL109
品牌：Imanis Life Sciences
产品描述
Species：Mouse
Cell Type：Melanoma
Transgene：Mouse sodium iodide symporter (mNIS) Near-infrared fluorescent protein (iRFP; ex/em 690/713 nm)
Selection Gene：Neomycin (Neo)；Puromycin (Puro) resistance
B16F10-mNIS-Neo/iRFP-Puro is a polyclonal population of the murine melanoma cell line B16F10 (ATCC® CRL-6475™). To achieve stable reporter expression in the polyclonal population, parental B16F10 cells were transduced with LV-mNIS-P2A-Neo (LV025) and LV-iRFP-P2A-Puro (LV032) and selected using G418 and puromycin. LV-mNIS-P2A-Neo (LV025) expresses the mouse sodium iodide symporter (mNIS) cDNA linked to the neomycin resistance gene (Neo) via a P2A self-cleavage peptide under the spleen focus-forming virus (SFFV) promoter. LV-iRFP-P2A-Puro expresses near-infrared fluorescent protein (iRFP) cDNA under the SFFV promoter and the puromycin resistance gene under the phosphoglycerate kinase promoter (PGK). *The ATCC trademark and trade name and any and all ATCC catalog numbers are trademarks of the American Type Culture Collection. This cell line has been tested for mycoplasma contamination and is certified mycoplasma free. The parental B16F10 cell line has been authenticated and certified free of interspecies cross contamination by short tandem repeat (STR) profiling with 9 STR loci. Due to the immunogenicity of the reporter genes in this cell line, we recommend using immunocompromised mice for in vivo studies.
