| 产品名称 | Imanis Life Sciences | CL066 | 4T1-mNIS-Puro/Fluc-Neo |
|---|---|
| 目录号 | CL066 |
| 别名 | 4T1-mNIS-Puro/Fluc-Neo |
| 外观 | 见COA |
| 分子量 | N/A |
| CAS | N/A |
| 溶解度 | |
| 存储条件 | 见COA |
| 保存时间 | 见COA |
| 备注1 | |
| 备注2 |
| 目录号 | 规格 | 价格 | 库存状态 | |
| CL066 | 咨询 | 咨询 |
Imanis Life Sciences | CL066 | 4T1-mNIS-Puro/Fluc-Neo
品名：4T1-mNIS-Puro/Fluc-Neo
货号：CL066
品牌：Imanis Life Sciences
产品描述
Species：Mouse
Cell Type：Mammary Carcinoma
Transgene：Mouse sodium iodide symporter (mNIS) Firefly luciferase (Fluc)
Selection Gene：Puromycin (Puro) Neomycin resistance (Neo)
4T1-mNIS-Puro/Fluc-Neo is a polyclonal population of the mouse mammary carcinoma cell line 4T1 (ATCC® CRL-2539™). To achieve stable reporter expression in the polyclonal population, parental 4T1 cells were transduced with LV-mNIS-PGK-Puro (LV022) and LV-Fluc-P2A-Neo (LV011) and selected using puromycin and G418. LV-mNIS-PGK-Puro encodes the mouse sodium iodide symporter (mNIS) cDNA under the spleen focus-forming virus (SFFV) promoter and the puromycin resistance gene (Puro) under the phosphoglycerate kinase (PGK) promoter. LV-Fluc-P2A-Neo (LV011) expresses firefly luciferase (Fluc) cDNA linked to the neomycin resistance gene (Neo) via a P2A self-cleavage peptide under the SFFV promoter. *The ATCC trademark and trade name and any and all ATCC catalog numbers are trademarks of the American Type Culture Collection. This cell line has been tested for mycoplasma contamination and is certified mycoplasma free. The parental 4T1 cell line has been authenticated and certified free of interspecies cross contamination by short tandem repeat (STR) profiling with 9 STR loci. Due to the immunogenicity of the reporter genes in this cell line, we recommend using immunocompromised mice for in vivo studies.
