| 产品名称 | Imanis Life Sciences | CL126 | 4T1-Fluc-Neo (monoclonal) |
|---|---|
| 目录号 | CL126 |
| 别名 | 4T1-Fluc-Neo (monoclonal) |
| 外观 | 见COA |
| 分子量 | N/A |
| CAS | N/A |
| 溶解度 | |
| 存储条件 | 见COA |
| 保存时间 | 见COA |
| 备注1 | |
| 备注2 |
| 目录号 | 规格 | 价格 | 库存状态 | |
| CL126 | 咨询 | 咨询 |
Imanis Life Sciences | CL126 | 4T1-Fluc-Neo (monoclonal)
品名：4T1-Fluc-Neo (monoclonal)
货号：CL126
品牌：Imanis Life Sciences
产品描述
Species：Mouse
Cell Type：Mammary Carcinoma
Transgene：Firefly Luciferase (Fluc)
Selection Gene：Neomycin resistance (Neo)
4T1-Fluc-Neo is a monoclonal population of the mouse mammary carcinoma cell line 4T1 (ATCC® CRL-2539™). To achieve stable reporter expression in the polyclonal population, parental 4T1 cells were transduced with LV-Fluc-P2A-Neo (LV011) and selected using G418. LV-Fluc-P2A-Neo encodes the firefly luciferase (Fluc) cDNA linked to the neomycin resistance gene (Neo) via a P2A cleavage peptide. A monoclonal population was isolated using three rounds of selection in a methylcellulose-based semi-solid medium. *The ATCC trademark and trade name and any and all ATCC catalog numbers are trademarks of the American Type Culture Collection. This cell line has been tested for mycoplasma contamination and is certified mycoplasma free. The parental 4T1 cell line has been authenticated and certified free of interspecies cross contamination by short tandem repeat (STR) profiling with 9 STR loci. Due to the immunogenicity of the reporter genes in this cell line, we recommend using immunocompromised mice for in vivo studies.
