| 产品名称 | Imanis Life Sciences | CL124 | PC3-Fluc-Neo/iRFP-Puro |
|---|---|
| 目录号 | CL124 |
| 别名 | PC3-Fluc-Neo/iRFP-Puro |
| 外观 | 见COA |
| 分子量 | N/A |
| CAS | N/A |
| 溶解度 | |
| 存储条件 | 见COA |
| 保存时间 | 见COA |
| 备注1 | |
| 备注2 |
| 目录号 | 规格 | 价格 | 库存状态 | |
| CL124 | 咨询 | 咨询 |
Imanis Life Sciences | CL124 | PC3-Fluc-Neo/iRFP-Puro
品名：PC3-Fluc-Neo/iRFP-Puro
货号：CL124
品牌：Imanis Life Sciences
产品描述
Species：Human
Cell Type：Prostate
Transgene：Firefly luciferase (Fluc) Near-infrared fluorescent protein (iRFP)
Selection Gene：Puromycin (Puro) Neomycin (Neo)
This is a polyclonal population derived from the prostate PC3 cell line (ATCC® CRL-1435TM). Parental PC3 cells were transduced with 1) LV-Fluc-P2A-Neo (Imanis #LV011) encoding the firefly luciferase (Fluc) cDNA under the spleen focus-forming virus (SFFV) promoter linked to the neomycin resistance gene (Neo) via a P2A cleavage peptide and 2) LV-iRFP-P2A-Puro (Imanis #LV032) encoding the near-infrared fluorescent protein (iRFP; ex/em = 690/713) cDNA under the spleen focus-forming virus (SFFV) promoter linked to the puromycin resistance gene (Puro) via a P2A cleavage peptide. High Fluc and iRFP expressing cells were selected using G418 and puromycin, respectively. *The ATCC trademark and trade name and any and all ATCC catalog numbers are trademarks of the American Type Culture Collection. This cell line has tested negative for mycoplasma contamination. The parental PC3 cell line has been authenticated and certified free of interspecies cross contamination by short tandem repeat (STR) profiling.
