| 产品名称 | Imanis Life Sciences | CL150 | Nalm6-Fluc-Neo/eGFP-Puro |
|---|---|
| 目录号 | CL150 |
| 别名 | Nalm6-Fluc-Neo/eGFP-Puro |
| 外观 | 见COA |
| 分子量 | N/A |
| CAS | N/A |
| 溶解度 | |
| 存储条件 | 见COA |
| 保存时间 | 见COA |
| 备注1 | |
| 备注2 |
| 目录号 | 规格 | 价格 | 库存状态 | |
| CL150 | 咨询 | 咨询 |
Imanis Life Sciences | CL150 | Nalm6-Fluc-Neo/eGFP-Puro
品名：Nalm6-Fluc-Neo/eGFP-Puro
货号：CL150
品牌：Imanis Life Sciences
产品描述
Species：Human
Cell Type：Lymphoma
Transgene：Firefly luciferase (Fluc) Enhanced green fluorescent protein (eGFP)
Selection Gene：Neomycin (neo) Puromycin resistance (puro)
Parental Nalm6 cells were transduced with 1) LV-Fluc-P2A-Neo (Imanis #LV011) encoding the firefly luciferase (Fluc) cDNA under the spleen focus-forming virus (SFFV) promoter and linked to the neomycin resistance gene (Neo) via a P2A cleavage peptide and 2) LV-eGFPPGK-Puro (Imanis #LV031) encoding the enhanced green fluorescent protein (eGFP) cDNA under the SFFV promoter and the puromycin resistance gene (Puro) under the phosphoglycerate kinase (PGK) promoter. A high Fluc and eGFP expressing population was generated by selection using G418 and puromycin followed by selection using a methylcellulose based semi-solid medium. The parental Nalm6 cell line was authenticated and certified free of interspecies cross contamination by STR profiling. This cell line has been tested for mycoplasma contamination and is certified mycoplasma free.
