| 产品名称 | Imanis Life Sciences | CL181 | Raji-Fluc-Puro/CD19-KO |
|---|---|
| 目录号 | CL181 |
| 别名 | Raji-Fluc-Puro/CD19-KO |
| 外观 | 见COA |
| 分子量 | N/A |
| CAS | N/A |
| 溶解度 | |
| 存储条件 | 见COA |
| 保存时间 | 见COA |
| 备注1 | |
| 备注2 |
| 目录号 | 规格 | 价格 | 库存状态 | |
| CL181 | 咨询 | 咨询 |
Imanis Life Sciences | CL181 | Raji-Fluc-Puro/CD19-KO
品名：Raji-Fluc-Puro/CD19-KO
货号：CL181
品牌：Imanis Life Sciences
产品描述
Species：Human
Cell Type：B lymphoblast
Transgene：Firefly luciferase (Fluc; Luc2)
Selection Gene：Puromycin resistance (Puro)
The Raji-Fluc-Puro/CD19-KO cell line stably expresses firefly luciferase and does not express CD19. To generate this cell line, Raji-Fluc-Puro (Imanis #CL161) cells were transfected with Cas9-gRNA ribonucleoprotein complexes targeting human CD19. Individual cell clones were generated using a methylcellulose based semi-solid medium and screened for CD19 expression by flow cytometry. Sequencing of the targeted CD19 region confirmed heterologous genetic knock-out in the selected cell clone.This cell line has been tested for mycoplasma contamination and is certified mycoplasma free.The parental Raji cell line was licensed and purchased directly from ATCC*.This cell line is also available for purchase as a pair with the Raji-Fluc-Puro (CD19 positive) cell line, for a discounted rate (link).*The ATCC trademark and trade name and any and all ATCC catalog numbers are trademarks of the American Type Culture Collection.
