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产品名称 Imanis Life Sciences | CL176 | U266B1-Fluc-Puro/eGFP-Neo
目录号 CL176
别名 U266B1-Fluc-Puro/eGFP-Neo
外观 见COA
分子量 N/A
CAS N/A
溶解度
存储条件 见COA
保存时间 见COA
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Imanis Life Sciences | CL176 | U266B1-Fluc-Puro/eGFP-Neo


品名:U266B1-Fluc-Puro/eGFP-Neo

货号:CL176

品牌:Imanis Life Sciences


产品描述

Species:Human

Cell Type:Multiple Myeloma

Transgene:Firefly luciferase (Fluc; Luc2);Emerald GFP (EmGFP)

Selection Gene:Puromycin;Neomycin

Parental U266B1 cells were transduced with LV-SFFV-Luc2-P2A-Puro (Imanis #LV012) and LV-SFFV-eGFP-P2A-Neo (Imanis #LV067), which encode the firefly luciferase (Fluc; Luc2) and enhanced green fluorescent protein (eGFP) cDNAs, respectively, under the spleen focus-forming virus (SFFV) promoter and linked to antibiotic resistance (puromycin, neomycin) genes via a P2A cleavage peptide. A high Fluc and eGFP expressing population was generated by selection using puromycin and G418 followed by selection with a methylcellulose based semi-solid medium.This cell line has been tested for Mycoplasma contamination and is certified mycoplasma free.The parental U266B1 cell line was licensed and purchased directly from ATCC*.*The ATCC trademark and trade name and any and all ATCC catalog numbers are trademarks of the American Type Culture Collection.



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