产品名称 | Imanis Life Sciences | CL176 | U266B1-Fluc-Puro/eGFP-Neo |
---|---|
目录号 | CL176 |
别名 | U266B1-Fluc-Puro/eGFP-Neo |
外观 | 见COA |
分子量 | N/A |
CAS | N/A |
溶解度 | |
存储条件 | 见COA |
保存时间 | 见COA |
备注1 | |
备注2 |
目录号 | 规格 | 价格 | 库存状态 | |
CL176 | 咨询 | 咨询 |
Imanis Life Sciences | CL176 | U266B1-Fluc-Puro/eGFP-Neo
品名:U266B1-Fluc-Puro/eGFP-Neo
货号:CL176
品牌:Imanis Life Sciences
产品描述
Species:Human
Cell Type:Multiple Myeloma
Transgene:Firefly luciferase (Fluc; Luc2);Emerald GFP (EmGFP)
Selection Gene:Puromycin;Neomycin
Parental U266B1 cells were transduced with LV-SFFV-Luc2-P2A-Puro (Imanis #LV012) and LV-SFFV-eGFP-P2A-Neo (Imanis #LV067), which encode the firefly luciferase (Fluc; Luc2) and enhanced green fluorescent protein (eGFP) cDNAs, respectively, under the spleen focus-forming virus (SFFV) promoter and linked to antibiotic resistance (puromycin, neomycin) genes via a P2A cleavage peptide. A high Fluc and eGFP expressing population was generated by selection using puromycin and G418 followed by selection with a methylcellulose based semi-solid medium.This cell line has been tested for Mycoplasma contamination and is certified mycoplasma free.The parental U266B1 cell line was licensed and purchased directly from ATCC*.*The ATCC trademark and trade name and any and all ATCC catalog numbers are trademarks of the American Type Culture Collection.