产品名称 | Imanis Life Sciences | CL174 | RPMI-8226-Fluc-Puro |
---|---|
目录号 | CL174 |
别名 | RPMI-8226-Fluc-Puro |
外观 | 见COA |
分子量 | N/A |
CAS | N/A |
溶解度 | |
存储条件 | 见COA |
保存时间 | 见COA |
备注1 | |
备注2 |
目录号 | 规格 | 价格 | 库存状态 | |
CL174 | 咨询 | 咨询 |
Imanis Life Sciences | CL174 | RPMI-8226-Fluc-Puro
品名:RPMI-8226-Fluc-Puro
货号:CL174
品牌:Imanis Life Sciences
产品描述
Species:Human
Cell Type:Multiple Myeloma
Transgene:Firefly luciferase (Fluc; Luc2)
Selection Gene:None
Parental RPMI-8226 cells were transduced with LV-SFFV-Luc2-P2A-Puro (Imanis #LV012) encoding the firefly luciferase (Fluc; Luc2) cDNA under the spleen focus-forming virus (SFFV) promoter and linked to the puromycin resistance gene (Puro) via a P2A cleavage peptide. A high Fluc expressing population was generated by selection using puromycin followed by selection with a methylcellulose based semi-solid medium.This cell line has been tested for Mycoplasma contamination and is free of Mycoplasma species.The parental RPMI-8226 cell line was licensed and purchased directly from ATCC*.The ATCC trademark and trade name and any and all ATCC catalog numbers are trademarks of the American Type Culture Collection.