产品名称 | DNA Polymerase Technology | GF100 | Lyoph-Ready Klentag1 |
---|---|
目录号 | |
别名 | Lyoph-Ready Klentag1 |
外观 | 见COA |
分子量 | N/A |
CAS | N/A |
溶解度 | |
存储条件 | 见COA |
保存时间 | 见COA |
备注1 | |
备注2 |
目录号 | 规格 | 价格 | 库存状态 | |
GF100 | 4000 rxns (equivalent to 200 ustandard enzyme. Volume may beup to 2.5x higher) | 咨询 | 咨询 |
DNA Polymerase Technology | GF100 | Lyoph-Ready Klentag1
品名:Lyoph-Ready Klentag1
货号:GF100
规格:4000 rxns (equivalent to 200 ustandard enzyme. Volume may beup to 2.5x higher)
品牌:DNA Polymerase Technology
产品描述
A reduced glycerol version of Klentaq1 suitable for lyophilization. Klentaq1 is named after Klenow fragment, and it is a similar N-terminal deletion (removing the 5'-[flap]exonuclease of Taq DNA polymerase). PCR tests show that Klentaq1 has improved fidelity (about 25%) and thermostability (about 2 degrees) relative to wild-type Taq, and it gives higher yields of amplicon. We speculate that the higher yields arise because at the end stages of a PCR reaction, the 5'-exonuclease can begin to attack the PCR product, but we are open to another opinion. Klentaq1 was used in the original formula for discovery of Long and Accurate PCR (Barnes, 1994; US patent 5,436,149). Klentaq1 also has inhibition resistance properties, tolerating 30 - 40% whole blood in a PCR reaction. Since Klentaq1 lacks the 5 prime exonuclease activity, it is not suitable for TaqMan assays.