产品名称 | DNA Polymerase Technology | HS290 | Hot Start CesiumKlentag C LA |
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目录号 | |
别名 | Hot Start CesiumKlentag C LA |
外观 | 见COA |
分子量 | N/A |
CAS | N/A |
溶解度 | |
存储条件 | 见COA |
保存时间 | 见COA |
备注1 | |
备注2 |
目录号 | 规格 | 价格 | 库存状态 | |
HS290 | 100ul(2000 x25ul rxns) | 咨询 | 咨询 |
DNA Polymerase Technology | HS290 | Hot Start CesiumKlentag C LA
品名:Hot Start CesiumKlentag C LA
货号:HS290
规格:100ul(2000 x25ul rxns)
品牌:DNA Polymerase Technology
产品描述
Hot Start Cesium Klentaq C LA is made with aptamer-based technology, enabling room temperature reaction set-up. Cesium Klentaq C is a cold-sensitive mutant of Klentaq1 (5'-exonuclease deficient Taq polymerase with improved fidelity and thermostability). Due to its suppressed activity at low temperatures this enzyme is designed for hot start PCR performance. Addition of the aptamer further enhances its hot start performance. The aptamer binds to the polymerase at sub-cycling temperatures, inactivating the enzyme and preventing spurious amplification. The Long-Accurate feature allows for amplification of longer products with higher fidelity and accuracy. LA enzymes are not recommended for use with dUTP. 10x buffer composition is: 500 mM Tris-Cl pH 9.2, 160 mM ammonium sulfate, 0.25% Brij 58, and 35 mM magnesium chloride.