产品名称 | DNA Polymerase Technology | HS100 | Hot Start Klentaq1 |
---|---|
目录号 | |
别名 | Hot Start Klentaq1 |
外观 | 见COA |
分子量 | N/A |
CAS | N/A |
溶解度 | |
存储条件 | 见COA |
保存时间 | 见COA |
备注1 | |
备注2 |
目录号 | 规格 | 价格 | 库存状态 | |
HS100 | 100 ul (2,000 X 25 ul rxns up to 1kb) | 咨询 | 咨询 |
DNA Polymerase Technology | HS100 | Hot Start Klentaq1
品名:Hot Start Klentaq1
货号:HS100
规格:100 ul (2,000 X 25 ul rxns up to 1kb)
品牌:DNA Polymerase Technology
产品描述
Hot Start Klentaq1 is made with aptamer-based technology, enabling room temperature reaction set-up. Klentaq1 is named after Klenow fragment, and it is a similar N-terminal deletion (removing the 5'-[flap]exonuclease of Taq DNA polymerase). PCR tests show that Klentaq1 has improved fidelity (about 25%) and thermostability (about 2 degrees) relative to wild-type Taq, and it gives higher yields of amplicon. We speculate that the higher yields arise because at the end stages of a PCR reaction, the 5'-exonuclease can begin to attack the PCR product, but we are open to another opinion. Klentaq1 was used in the original formula for discovery of Long and Accurate PCR (Barnes, 1994; US patent 5,436,149). Klentaq1 also has inhibition resistance properties, tolerating 30 - 40% whole blood in a PCR reaction.