收缩
收缩
| 货号： | G1780 | 规格： | 1000 assays |
| 品牌： | 热销产品 | 价格： | ￥ |
| 产品分类： | 特价奥特莱斯专区 | 研究领域： | |
| CAS编号： | 分子量： | ||
| 分子式： | 别名： | ||
| 储存条件： | 运输条件： | ||
| 货期： | 应用： | ||
| 说明书： | 备注： | ||
| 产品描述 | |||
|
维百奥生物库存现货，特价销售CytoTox 96® Non-Radioactive Cytotoxicity Assay G1780！ | 产品信息 品名：CytoTox 96® Non-Radioactive Cytotoxicity Assay G1780 品牌：Promega 货号：G1780 规格：1000 assays | 产品描述 The CytoTox 96® Non-Radioactive Cytotoxicity Assay is a colorimetric alternative to radioactive cytotoxicity assays. The assay quantitatively measures lactate dehydrogenase (LDH), a stable cytosolic enzyme that is released upon cell lysis, in much the same way as [51Cr] is released in radioactive assays. Released LDH in culture supernatants is measured with a 30-minute coupled enzymatic assay that results in the conversion of a tetrazolium salt (INT) into a red formazan product. The amount of color formed is proportional to the number of lysed cells. Visible wavelength absorbance data are collected using a standard 96-well plate reader. The assay can be used to measure membrane integrity for cell-mediated cytotoxicity assays in which a target cell is lysed by an effector cell, or to measure lysis of target cells by bacteria, viruses, proteins, chemicals, etc. The CytoTox 96® Non-Radioactive Cytotoxicity Assay requires no radioactive waste disposal or [51Cr] and eliminates labeling of target cells prior to conducting experiments. Standard equipment can be used to collect absorbance (visible wavelength) data with a standard 96-well plate reader. The assay can be used for a variety of applications including measurement of 1) cell-mediated cytotoxicity; 2) chemical-mediated cytotoxicity; and 3) total cell number. A highly sensitive assay, it can reveal early, low-level damage to cell membranes that is often missed with other methodologies. | 产品特点 · Measures release of LDH, an indicator of cytotoxicity · Non-radioactive, time-saving assay |
|||
