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Name ：ZymoBIOMICS Microbial Community Standard II (Log Distribution)

Cat # ：D6310    

Size ：10 Preps

Brand：Zymo Research

_d6310_zymobiomics_microbial_community_standard_ii_(log_distribution).pdf

HIGHLIGHTS

Log abundance distribution: assess detection limit over a broad range (102 to 108 cells)

Accurate composition: assess the accuracy of microbiome measurements

Microbiomics QC: ideal for quality control of microbiome measurements

DESCRIPTION

ZymoBIOMICS Microbial Community Standard II (Log Distribution) is a mock microbial community consisting of eight bacterial and two fungal strains. This microbial standard can be used to assess the performance of microbiomics workflows and can also be used as a positive control for routine sequencing. Cells of the ten microbes were mixed in a log-distributed abundance, which allows the user to easily assess the detection limit of their microbiomics workflow. 75 µl of the standard contains about ~100 cells of Staphylococcus aureus, the organism of lowest abundance. If needed, the standard can be spiked into a sample matrix (e.g. soil and blood) to mimic real samples of interest. Theoretical Composition Based on Genomic DNA: Listeria monocytogenes - 89.1%, Pseudomonas aeruginosa - 8.9%, Bacillus subtilis - 0.89%, Saccharomyces cerevisiae - 0.89%, Escherichia coli - 0.089%, Salmonella enterica - 0.089%, Lactobacillus fermentum - 0.0089%, Enterococcus faecalis - 0.00089%, Cryptococcus neoformans - 0.00089%, and Staphylococcus aureus - 0.000089%.

TECHNICAL SRECIFICATIONS

Applicable For

NGS, microbiomics, metagenomics.

Processing Volume

75 µl per preparation.

Purity

Contains < 0.01% foreign microbial DNA.

Sample Source

Eight bacteria (3 Gram-negative and 5 Gram-positive) and 2 yeasts.

Yield

Approximately 220 ng DNA per preparation.