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Frozen-EZ Yeast Transformation II Kit

Cat #：T2001

Brand：Zymo Research

Size：120 Rxns

_t2001_frozen-ez_yeast_transformation_ii.pdf

HIGHLIGHTS

Fast: Yeast cells with high transformation efficiencies can be prepared in under 10 minutes.

Simple: Easy method to transform with single or multiple plasmids in ≤ 1 hour with carrier DNA.

Versatile: Can be used with S. cerevisiae, as well as other fungi, including C. albicans, S. pombe, and P. pastoris. Compatible with both circular and linear DNA.

DESCRIPTION

The Frozen-EZ Yeast Transformation II Kit is designed to make yeast transformations and library screening easier and more efficient than currently available methods. The yeast cells prepared with the Frozen-EZ Yeast Transformation II Kit can be used immediately for transformation or can be stored (i.e., ≤ -70°C) for use at a later time. Yeast prepared with this kit can be transformed with both circular and linear DNAs. Also, the Frozen-EZ Yeast Transformation II Kit can be used with other fungi including C. albicans, S. pombe, and P. pastors.

TECHNICAL SRECIFICATIONS

Competent Cell Stability  

≥ 1 year at -70°C

Processing Time

≤ 15 minutes

Product Storage

0 - 4°C

Transformation DNA Input

0.2 - 1.0 µg

Transformation Efficiency

10⁵ - 10⁶ cfu/µg plasmid DNA.