维百奥生物代理TopoGEN品牌全系列产品
TopoGEN是一家领先的科研诊断公司，专注于为癌症研究领域提供高质量的拓扑异构酶和DNA旋转酶。核心产品包括：药物筛选试剂盒，用于鉴定适用于癌症化疗和抗菌应用的拓扑异构酶活性药物；检测试剂盒，用于体外和体内检测原核及真核拓扑异构酶（I型、II型、IV型、旋转酶）；全系列产品线，包含DNA底物与DNA裂解靶点、拓扑异构酶抑制剂、拓扑异构酶抗体及试剂盒补充试剂产品。同时TopoGEN还提供合同筛选服务，全程在内部开发和进行基于机制的强大定制化筛选，其细胞筛选平台可检测癌细胞环境中的拓扑异构酶I和II毒物，代表了发现新型抗癌药物的全新金标准。
▍TopoGEN产品线
● Topo Enzymes：包含人类拓扑I、突变人类拓扑I、人类拓扑IIα以及大肠杆菌拓扑IV。
● DNA旋转酶：
ⅰ.大肠杆菌DNA旋转酶:高度纯化的大肠杆菌 DNA 旋转酶以全酶形式提供，是一种出色的试剂，可用于体外超螺旋质粒或用于新药筛选。
ⅱ.金黄色葡萄球菌DNA旋转酶：高度纯化的金黄色葡萄球菌 DNA 旋转酶是一种II型拓扑异构酶，由来自金黄色葡萄球菌的两个基因 GyrA 和 GyrB 编码。DNA旋转酶是一种必需的拓扑异构酶，它通过链断裂/重新密封和DNA包裹的过程使DNA超螺旋。作为一种II 型酶，旋转酶的独特之处在于它能够对松弛的质粒DNA底物进行负超螺旋。金黄色葡萄球菌DNA旋转酶也是基于喹诺酮类药物的抗菌剂的靶标，该抗菌剂通过将酶分解为DNA损伤剂来发挥作用。金黄色葡萄球菌酶是GyrA2GyrB2的异四聚体，并被纯化为His标记的亚基，这些亚基被重新组装以产生活性酶。
● 拓扑套件：包括Topol检测试剂盒、Topoll检测试剂盒、旋转酶检测试剂、DNA 放卷试剂盒、旋转酶和松弛DNA 检测试剂盒、TopI-NuEx 试剂盒、TopI-NucEx 试剂盒。
● 药物筛选试剂盒：可用于明确识别拓扑异构酶靶向分子，用于癌症化疗和抗菌应用。包括拓扑I、拓扑二、Topo ll(基于kDNA)、拓扑四、旋转酶、Topol高负载抑制试剂盒。
● Topo抗体以及蛋白质Mw标记物和抗体试剂盒。
● DNA底物：为拓扑异构酶相关实验提供特定序列和结构的DNA底物，以便研究拓扑异构酶对不同DNA底物的作用机制。
● TDP1产品和TDP2产品：参与DNA修复过程的酶，相关产品可用于DNA修复机制研究以及开发针对这些酶的药物。
● 拓扑相关药物：提供已知的拓扑异构酶抑制剂等相关药物，用于科研实验对照、药物作用机制研究等。
● 用于DNA修复检测的稳定细胞系：为研究DNA修复过程提供稳定的细胞模型，科研人员可以利用这些细胞系研究拓扑异构酶在DNA修复中的作用，以及评估药物对DNA修复功能的影响
● 基于转染的DNA修复检测：基于转染的HR和NHEJ试剂盒使用瞬时转染来制作含有DNA报告基因的细胞库，使研究者能够询问2种主要的DNA修复途径。
▍TopoGEN产品列表
| 货号 | 品名 | 规格 |
| TG2000EC | E. coli Topoisomerase IV Enzyme | 250 Units; 500 Units; 1000 Units |
| TG2005HRC | Human Topoisomerase I Enzyme | 250 Units; 500 Units; 3000 Units |
| TG2000H | Human Topoisomerase IIα Enzyme | 250 Units; 500 Units; 1000 Units; 2000 Units |
| TG2005H-Y723F | Mutant Human Topoisomerase I | 10ug |
| TG2000G | E. Coli DNA Gyrase Enzyme | 100 Units; 500 Units; 1000 Units; 2000 Units |
| TG2000GSA | Staph aureus DNA Gyrase Enzyme | 100 Units; 500 Units; 1000 Units |
| TG1015 | Human Topoisomerase I Assay Kit | 100 Assays; 250 Assays; 500 Assays |
| TG1001 | Human Topoisomerase II Assay Kit | 100 Assays; 250 Assays; 500 Assays |
| TG1003 | Gyrase Drug Screening Kit (kDNA Based) | 1 kit |
| TG1025 | DNA Intercalator/Unwinding Kit | 1 kit |
| TG2000GKIT | E. coli DNA Gyrase and Relaxed DNA Assay Kit | 100 Units; 500 Units; 1000 Units; 2000 Units |
| TG1040-1 | TopI-NucEx Kit | 1 kit |
| TG1050-1 | TopII-NucEx Kit | 1 kit |
| TG2001GKIT | E. coli DNA Gyrase Drug Screening Kit (Plasmid Based) | 100 Assays; 500 Assays; 1000 Assays; 2000 Assays |
| TG1007 | E. coli Topoisomerase IV Drug Screening Kit | 100 Assays; 250 Assays; 100 Assays |
| TG1018 | Topoisomerase I Drug Screening Kit | 100 Assays; 250 Assays; 100 Assays |
| TG1017 | Topoisomerase I Hi-Loading Inhibition Kit | 500 Units; 1000 Units |
| TG1009 | Topoisomerase II Drug Screening Kit | 100 Assays; 250 Assays; 100 Assays |
| TG1019 | Topoisomerase II Drug Screening Kit (kDNA based) | 100 Assays; 250 Assays; 100 Assays |
| TG2012-4 | Monoclonal Antibody to Human Topoisomerase I | - |
| TG2012 | Polyclonal Antibody to Human Topoiosomerase I (Scleroderma Patient Serum) | - |
| TG2012-2 | Polyclonal to Human Topoisomerase I (serum) | - |
| TG2011-5 | Monoclonal Antibody to Human Topoiosomerase II Beta | - |
| TG2010-1 | Monoclonal Antibody to Human Topoisomerase II Alpha | - |
| TG2010-3 | Polyclonal Antibody to Human Topoiosomerase II Beta | - |
| TG2011-1 | Polyclonal Antibody to Human Topoisomerase II Alpha | - |
| TG2014 | Polyclonal Antibody to Yeast Topoisomerase II (Serum) | - |
| TG2011-2 | Topoisomerase II Alpha C-Terminal Peptide | - |
| TG2010-4 | Polyclonal Antibody to Human Topoisomerase III Beta | - |
| TG2015 | Monoclonal Antibody to Human Topoisomerase III Alpha | - |
| TG1020 | Human Topoisomerase ICE Assay Kit | 24 tests |
| TG2011-3 | Human Topoisomerase II Alpha Molecular Weight Marker | - |
| TG2011-4 | Topoisomerase I Western Blot Marker | - |
| TG1030 | Topoisomerase II Immunoflourescence Kit | 50 ug |
| TG1035 | Topoisomerase II Immunoprecipitation Kit | 100 ul |
| TG1024 | Topoisomerase II Band Shift Kit | 25 Assays |
| TG2013 | Kinetoplast DNA (kDNA) | 25 ug; 50 ug; 250 ug |
| TG2018-1 | Linear kDNA | - |
| TG2020-1 | Decatenated kDNA | - |
| TG2030 | Supercoiled pHOT-1 Plasmid DNA Substrate | 25 ug; 50 ug; 250 ug; 500 ug |
| TG2035 | Relaxed pHOT-1 Plasmid DNA Substrate | 25 ug; 50 ug; 250 ug; 500 ug |
| TG2004H | Human TDP-1 | 250 Units; 500 Units |
| TG2016 | Polyclonal Antibody to Human TDP-1 | 250 units |
| TG1004 | TDP-1 Assay Kit | 100 Assays; 250 Assays |
| TG2003H | Human TDP-2 | 25 ug; 50 ug |
| TG1005 | Human TDP-2 Assay Kit | 100 Assays; 250 Assays |
| TG2038 | Tyrosyl DNA Phosphodiesterase 2 (TDP-2) Substrate | 10 ug; 50 ug |
| TG4103 | 10-Hydroxy Camptothecin | - |
| TG4104 | 9-Amino Camptothecin | - |
| TG4102 | 9-Nitro-20 (S) Camptothecin (Rubitecan) | - |
| TG4110 | Camptothecin | - |
| TG4100 | Topotecan Hydrochloride (Hycamtin) | - |
| TG4150 | Amsacrine (m-AMSA) | - |
| TG4130 | Daunomycin | - |
| TG4120 | Doxorubicin (Adriacin) | - |
| TG4170 | Ellipticine | - |
| TG4140 | Etoposide (VP16) | - |
| TG4160 | Genistein | - |
| TG4200 | HU-331 | - |
| TG4180 | Ciprofloxacin | 50 ul |
| DR-3050 | Homologous Recombination (HR) Repair Reporter Kit | 50 ug; 250 ug; 500 ug; 1000 ug |
| DRT-5050 | Non-Homologous End Joining (NHEJ) Repair Reporter Kit | 50 ug; 250 ug; 500 ug; 1000 ug |
| DR3000-HRHeLa-Sce | HeLa Cell HR Kit | 25 Assays; 50 Assays; 125 Assays |
| DR3000-HRHeLa | HeLa Cell HR Kit | 25 Assays; 50 Assays; 125 Assays |
| DR3000-HRSH-Sce | Human: Neuroblastoma HR Reporter Cell line | 25 Assays; 50 Assays; 125 Assays |
| DR3000-HR3T3-Sce | Mouse: 3T3 HR Reporter Cell Line | 25 Assays; 50 Assays; 125 Assays |
| DR3000-HRCN1.4-Sce | Mouse: Neuronal Lineage HR Reporter Cell line | 25 Assays; 50 Assays; 125 Assays |
| DR3000 | Custom Cell Based Screening Kit for (HR) Pathway | 25 Assays; 50 Assays; 125 Assays |
| DR5001-HNHeLa-Sce | Human:HeLa NHEJ Reporter Cell Line | 25 Assays; 50 Assays; 125 Assays |
| DR5000-HNSH-Sce | Human Neuroblastoma cell HNSH NHEJ Reporter with I-Sce1 DNA | 25 Assays; 50 Assays; 125 Assays |
| DR3050 | Homologous Recombination (HR) Repair Reporter Kit | 50 ug; 250 ug; 500 ug; 1000 ug |
| DRT5050 | Homologous Recombination (HR) Repair Reporter Kit | 50 ug; 250 ug; 500 ug; 1000 ug |
| TG4045 | S. Aureus Gyrase Assay Buffer | - |
| TG4040 | Topoisomerase II Assay Buffer | - |
| TG4095 | Topoisomerase I Dilution Buffer | - |
| TG4035 | 20mM ATP | - |
| TG4037 | 5X Universal Stop Buffer | - |
| TG4060 | 10% Sodium Dodecyl Sulfate | - |
| TG4038 | 10X Gel Loading Buffer | - |
| TG4065 | 20% Sarkosyl | - |
| TG4030 | E. coli DNA Gyrase Assay Buffer | - |
| TG4039 | Proteinase K | - |
| TG4090 | Topoisomerase II Dilution Buffer | - |
| TG4032 | Topoisomerase IV Assay Buffer | - |
| TG4055 | Potassium Glutamate | - |
